Antibody was affinity purified using an epitope specific to FIGNL1 immobilized on solid support. The epitope recognized by A300-773A maps to a region between residue 325 and 375 of human Fidgetin-Like 1using the numbering given in entry NP_071399.2 (GeneID 63979). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
fidgetin-like protein 1
between 325 and 375
2 - 8°C
Detection of human FIGNL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-FIGNL1 antibody BL2229 (Cat. No. A300-768A) used for IP at 3 µg/mg lysate. FIGNL1 was also immunoprecipitated by rabbit anti-FIGNL1 antibodies BL2227 (Cat. No. A300-772A), BL2228 and BL2230. For blotting immunoprecipitated FIGNL1, BL2227 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.