Antibody was affinity purified using an epitope specific to FIGNL1 immobilized on solid support. The epitope recognized by A300-772A maps to a region between residue 1 and 50 of human Fidgetin-Like 1using the numbering given in entry NP_071399.2 (GeneID 63979). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
fidgetin-like protein 1
between 1 and 50
2 - 8°C
Detection of human and mouse FIGNL1 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-FIGNL1 antibody BL2227 (Cat. No. A300-772A) used for WB at 1 µg/ml (A and B) and used for IP at 3 µg/mg lysate (B). FIGNL1 was also immunoprecipitated by rabbit anti-FIGNL1 antibodies BL2228, BL2229 (Cat. No. A300-773A) and BL2230. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).