Antibody was affinity purified using an epitope specific to HEC1 immobilized on solid support. The epitope recognized by A300-771A maps to a region between residue 600 and the C-terminus (residue 642) of human Highly Expressed in Cancer 1 (Kinetochore Associated 2) using the numbering given in entry NP_006092.1 (GeneID 10403). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
HEC, HEC1, highly expressed in cancer protein, highly expressed in cancer, rich in leucine heptad repeats, HsHec1, hsNDC80, kinetochore associated 2, kinetochore protein Hec1, kinetochore protein NDC80 homolog, kinetochore-associated protein 2, KNTC2, NDC
between 600 and C-term
2 - 8°C
Detection of human and mouse HEC1 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HEC1 antibody BL3443 (Cat. No. A300-771A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). HEC1 was also immunoprecipitated by rabbit anti-HEC1 antibodies BL3440 (Cat. No. A300-770A) and BL3441. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).