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Rabbit anti-HEC1 Antibody, Affinity Purified

Catalogue number:
A300-771A
Size:
20 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to HEC1 immobilized on solid support. The epitope recognized by A300-771A maps to a region between residue 600 and the C-terminus (residue 642) of human Highly Expressed in Cancer 1 (Kinetochore Associated 2) using the numbering given in entry NP_006092.1 (GeneID 10403). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

HEC, HEC1, highly expressed in cancer protein, highly expressed in cancer, rich in leucine heptad repeats, HsHec1, hsNDC80, kinetochore associated 2, kinetochore protein Hec1, kinetochore protein NDC80 homolog, kinetochore-associated protein 2, KNTC2, NDC

Species Reactivity:

Human

Immunogen:

between 600 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse HEC1 by WB

Detection of human and mouse HEC1 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HEC1 antibody BL3443 (Cat. No. A300-771A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). HEC1 was also immunoprecipitated by rabbit anti-HEC1 antibodies BL3440 (Cat. No. A300-770A) and BL3441. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).