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Rabbit anti-HEC1 Antibody, Affinity Purified - A300-771A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-HEC1 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-771A
Name:
Rabbit anti-HEC1 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to HEC1 immobilized on solid support.The epitope recognized by A300-771A maps to a region between residue 600 and the C-terminus (residue 642) of human Highly Expressed in Cancer 1 (Kinetochore Associated 2) using the numbering given in entry NP_006092.1 (GeneID 10403).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
HEC, HEC1, highly expressed in cancer protein, highly expressed in cancer, rich in leucine heptad repeats, HsHec1, hsNDC80, kinetochore associated 2, kinetochore protein Hec1, kinetochore protein NDC80 homolog, kinetochore-associated protein 2, KNTC2, NDC
Species Reactivity:
Human
Immunogen:
Between 600 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse HEC1 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HEC1 antibody BL3443 (Cat. No. A300-771A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). HEC1 was also immunoprecipitated by rabbit anti-HEC1 antibodies BL3440 (Cat. No. A300-770A) and BL3441. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

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