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Rabbit anti-TORC1 Antibody, Affinity Purified

Catalogue number:
A300-768A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to TORC1 immobilized on solid support. The epitope recognized by A300-768A maps to a region between residue 25 and 75 of human Transducer of Regulated cAMP Response element-binding protein 1 (CREB Regulated Transcription Coactivator 1) using the numbering given in entry NP_056136.1 (GeneID 23373 ). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP
Product Type:

Antibodies

Alternative Names:

CREB-regulated transcription coactivator 1, Mam-2, MAML2, Mastermind-like protein 2, MECT1, mucoepidermoid carcinoma translocated protein 1, TORC1, TORC-1, Transducer of CREB protein 1, transducer of regulated cAMP response element-binding protein (CREB)

Species Reactivity:

Human

Immunogen:

between 25 and 75

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human TORC1 by western blot

Detection of human TORC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TORC1 antibody BL3431 (Cat. No. A300-768A) used for IP at 3 µg/mg lysate. TORC1 was also immunoprecipitated by rabbit anti-TORC1 antibodies BL3433 and BL3434 (Cat. No. A300-769A). For blotting immunoprecipitated TORC1, BL3434 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.