Antibody was affinity purified using an epitope specific to TORC1 immobilized on solid support. The epitope recognized by A300-768A maps to a region between residue 25 and 75 of human Transducer of Regulated cAMP Response element-binding protein 1 (CREB Regulated Transcription Coactivator 1) using the numbering given in entry NP_056136.1 (GeneID 23373 ). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CREB-regulated transcription coactivator 1, Mam-2, MAML2, Mastermind-like protein 2, MECT1, mucoepidermoid carcinoma translocated protein 1, TORC1, TORC-1, Transducer of CREB protein 1, transducer of regulated cAMP response element-binding protein (CREB)
between 25 and 75
2 - 8°C
Detection of human TORC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TORC1 antibody BL3431 (Cat. No. A300-768A) used for IP at 3 µg/mg lysate. TORC1 was also immunoprecipitated by rabbit anti-TORC1 antibodies BL3433 and BL3434 (Cat. No. A300-769A). For blotting immunoprecipitated TORC1, BL3434 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.