Rabbit anti-Phospho KAP-1 (S824) Antibody, Affinity Purified - A300-767A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Detection of human Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (15 and 50 µg) from HEK293T cells treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody A300-767A (lot A300-767A-4) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel shows western blot for total KAP1-1 using affinity purified rabbit anti-KAP-1 antibody A300-274A at 0.1 µg/ml with an exposure time of 30 seconds.

Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 cells treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody A300-767A (lot A300-767A-4) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Phospho-KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-phospho-KAP-1 (S824) (A300-767A lot 4) and rabbit anti-KAP-1 antibody A300-274A used for IP at 3 µg/mg lysate. For blotting immunoprecipitated Phospho-KAP-1 (S824), A300-767A lot 4 was used at 1 µg/ml. To examine total KAP-1, the blot was stripped and then blotted with A300-274A at 1 µg/ml (lower panel). Detection: Chemiluminescence with an exposure time of 10 seconds (upper) and 3 seconds (lower).

Detection of human Phospho KAP-1 (S824) by immunocytochemistry. Samples: FFPE serial sections of asynchronous HeLa cells treated with etoposide (left) and untreated HeLa cells (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) (Cat. No. A300-767A Lot4) used at a dilution of 1:200 (1µg/ml). Detection: DAB

Detection of human Phospho Kap1 (S824) by immunohistochemistry. Sample: FFPE serial section of human lung cancer (Cat. No. A300-767A Lot4) used at a dilution of 1:200 (1 µg/ml). Detection: DAB

Detection of human Phospho KAP-1 (S824) by immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with etoposide (left) or untreated (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) (Cat. No. A300-767A Lot3) used at a dilution of 1:200 (1µg/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight® 594 conjugated used at a dilution of 1:100.