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  3. A300-767A
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Rabbit anti-Phospho KAP-1 (S824) Antibody, Affinity Purified

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A300-767A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µl (200 µg/ml) _$$_
Product is available in:
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£464.00
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Estimated delivery Fri 26 Apr
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Applications:

WB, IP, IHC, ICC, ICC-IF

Antibody Host:

Rabbit

Target:

KAP-1, Phospho (S824)

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

surrounding serine 824

Alternative Names:

KRAB [Kruppel-associated box domain]-associated protein 1; RING finger protein 96; Tripartite motif-containing protein 28; transcriptional intermediary factor 1-beta; transcription intermediary factor 1-beta; TIF1-beta; TIF1B; TF1B; RNF96; RING-type E3 ub

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Product Description:

Rabbit anti-Phospho KAP-1 (S824) Antibody, Affinity Purified - 100 µl (200 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human Phospho KAP-1 (S824)
Detection of human Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (15 and 50 µg) from HEK293T cells treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody A300-767A (lot A300-767A-4) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel shows western blot for total KAP1-1 using affinity purified rabbit anti-KAP-1 antibody A300-274A at 0.1 µg/ml with an exposure time of 30 seconds.
Detection of mouse Phospho KAP-1 (S824)
Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 cells treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody A300-767A (lot A300-767A-4) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human Phospho-KAP-1 (S824)
Detection of human Phospho-KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-phospho-KAP-1 (S824) (A300-767A lot 4) and rabbit anti-KAP-1 antibody A300-274A used for IP at 3 µg/mg lysate. For blotting immunoprecipitated Phospho-KAP-1 (S824), A300-767A lot 4 was used at 1 µg/ml. To examine total KAP-1, the blot was stripped and then blotted with A300-274A at 1 µg/ml (lower panel). Detection: Chemiluminescence with an exposure time of 10 seconds (upper) and 3 seconds (lower).
Detection of human Phospho KAP-1 (S824)
Detection of human Phospho KAP-1 (S824) by immunocytochemistry. Samples: FFPE serial sections of asynchronous HeLa cells treated with etoposide (left) and untreated HeLa cells (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) (Cat. No. A300-767A Lot4) used at a dilution of 1:200 (1µg/ml). Detection: DAB
Detection of human Phospho Kap1 (S824) b
Detection of human Phospho Kap1 (S824) by immunohistochemistry. Sample: FFPE serial section of human lung cancer (Cat. No. A300-767A Lot4) used at a dilution of 1:200 (1 µg/ml). Detection: DAB
Detection of human Phospho KAP-1 (S824)
Detection of human Phospho KAP-1 (S824) by immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with etoposide (left) or untreated (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) (Cat. No. A300-767A Lot3) used at a dilution of 1:200 (1µg/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight® 594 conjugated used at a dilution of 1:100.
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