Antibody was affinity purified using an epitope specific to E2F1 immobilized on solid support. The epitope recognized by A300-766A maps to a region between residue 387 and 437 of human E2F Transcription Factor 1 using the numbering given in entry NP_005216.1 (GeneID 1869). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
E2F-1, PBR3, PRB-binding protein E2F-1, RBAP1, RBAP-1, RBBP3, RBBP-3, RBP3, retinoblastoma-associated protein 1, retinoblastoma-binding protein 3, transcription factor E2F1
between 387 and 437
2 - 8°C
Detection of human E2F1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50µg) cells. Antibodies: Affinity purified rabbit anti-E2F1 antibody BL3390 (Cat. No. A300-766A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). E2F1 was also immunoprecipitated using rabbit anti-E2F1 antibody BL3388 (Cat. No. A300-765A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).