Antibody was affinity purified using an epitope specific to E2F1 immobilized on solid support. The epitope recognized by A300-765A maps to a region between residue 300 and 350 of human E2F Transcription Factor 1 using the numbering given in entry NP_005216.1 (GeneID 1869). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
E2F-1, PBR3, PRB-binding protein E2F-1, RBAP1, RBAP-1, RBBP3, RBBP-3, RBP3, retinoblastoma-associated protein 1, retinoblastoma-binding protein 3, transcription factor E2F1
between 300 and 350
2 - 8°C
Detection of human E2F1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-E2F1 antibody BL3388 (Cat. No. A300-765A) used for IP at 3 µg/mg lysate. E2F1 was also immunoprecipitated using rabbit anti-E2F1 antibody BL3390 (Cat. No. A300-766A) at 3 µg/mg lysate. For blotting immunoprecipitated E2F1, BL3390 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human E2F1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-E2F1 (Cat. No. A300-765A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB