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Rabbit anti-Atrophin 1 Antibody, Affinity Purified - A300-753A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Atrophin 1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-753A
Name:
Rabbit anti-Atrophin 1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Atrophin 1 immobilized on solid support.The epitope recognized by A300-753A maps to a region between residue 1150 and the C-terminus (residue 1190) of human Atrophin 1 using the numbering given in entry NP_001007027.1 (GeneID 1822 ).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
atrophin-1, B37, D12S755E, dentatorubral-pallidoluysian atrophy protein, DRPLA, HRS, NOD
Species Reactivity:
Human
Immunogen:
Between 1150 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Atrophin 1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Atrophin 1 antibody A300-753A (lot A300-753A-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human Atrophin 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Atrophin 1 antibody A300-753A (lot A300-753A-2) used for IP at 3 µg per reaction. Atrophin 1 was also immunoprecipitated by rabbit anti-Atrophin 1 antibody BL3347. For blotting immunoprecipitated Atrophin 1, A300-753A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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