Antibody was affinity purified using an epitope specific to DHX29 immobilized on solid support. The epitope recognized by A300-751A maps to a region between residue 150 and 200 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 29 using the numbering given in entry NP_061903.2 (GeneID 54505). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent RNA helicase DHX29, DDX29, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 29, DEAH (Asp-Glu-Ala-His) box polypeptide 29, DEAH box protein 29, DEAH-box helicase 29, nucleic acid helicase DDXx
between 150 and 200
2 - 8°C
Detection of human DHX29 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DHX29 antibody BL3241 (Cat. No. A300-751A) used for WB at 1 µg/ml (A and B) and used for IP at 3 µg/mg lysate (B). DHX29 was also immunoprecipitated using rabbit anti-DHX29 antibody BL3240 (Cat. No. A300-750A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).