Antibody was affinity purified using an epitope specific to RCD8 immobilized on solid support. The epitope recognized by A300-746A maps to a region between residue 1351 and 1401 of human RCD8 Autoantigen using the numbering given in entry AAH43616 (GeneID 23644). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
autoantigen Ge-1, autoantigen RCD-8, enhancer of mRNA-decapping protein 4, GE1, Ge-1, HEDL5, HEDLS, human enhancer of decapping large subunit, RCD8, RCD-8
between 1351 and 1401
2 - 8°C
Detection of human RCD8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RCD8 antibody A300-746A used for IP at 3 µg/mg lysate. RCD8 was also immunoprecipitated by rabbit anti-RCD8 antibody A300-745A. For blotting immunoprecipitated RCD8, A300-745A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.