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Rabbit anti-RCD8 Antibody, Affinity Purified

Catalogue number:
A300-745A
Size:
20 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to RCD8 immobilized on solid support. The epitope recognized by A300-745A maps to a region between residue 1 and 50 of human RCD8 Autoantigen using the numbering given in entry AAH43616 (GeneID 23644). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

autoantigen Ge-1, autoantigen RCD-8, enhancer of mRNA-decapping protein 4, GE1, Ge-1, HEDL5, HEDLS, human enhancer of decapping large subunit, RCD8, RCD-8

Species Reactivity:

Human, Mouse

Immunogen:

between 1 and 50

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human RCD8 by western blot.

Detection of human RCD8 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCD8 antibody A300-745A (lot A300-745A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of mouse RCD8 by western blot.

Detection of mouse RCD8 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCD8 antibody A300-745A (lot A300-745A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human RCD8 by western blot

Detection of human RCD8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RCD8 antibody A300-745A (lot A300-745A-2) used for IP at 6 µg per reaction. RCD8 was also immunoprecipitated by a previous lot of this antibody (lot A300-745A-1). For blotting immunoprecipitated RCD8, A300-745A was used at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.