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Rabbit anti-SRA Antibody, Affinity Purified

Catalogue number:
A300-743A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to SRA immobilized on solid support. The epitope recognized by A300-743A maps to a region between residue 180 and the C-terminus (residue 237) of human Steroid Receptor RNA Activator 1 using the numbering given in entry NP_001030312.1 (GeneID 10011). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Detection of human SRA by western blot a
Product Type:

Antibodies

Alternative Names:

pp7684, SRA, SRAP, steroid receptor coactivator, steroid receptor RNA activator 1, steroid receptor RNA activator 1 (complexes with NCOA1), steroid receptor RNA activator protein, STRAA1

Species Reactivity:

Human

Immunogen:

between 180 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human SRA by western blot a

Detection of human SRA by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (20 and 4 µg for WB; 1 mg for IP, 20% of IP loaded), MCF-7 (7; 20 µg) and HEK293T (T; 20µg). Antibodies: Affinity purified rabbit anti-SRA antibody A300-743A (lot A300-743A-1) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). SRA was also immunoprecipitated using rabbit anti-SRA antibody BL2734 at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 5 minutes (A) and 10 seconds (B).