Antibody was affinity purified using an epitope specific to HMG2a immobilized on solid support. The epitope recognized by A300-736A maps to a region between residue 125 and 175 of human High-Mobility Group 2A using the numbering given in entry NP_005333.2 (GeneID 3149). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
high mobility group protein 2a, High mobility group protein 4, high mobility group protein B3, high-mobility group (nonhistone chromosomal) protein 4, HMG2A, HMG-2a, HMG4, HMG-4
between 125 and 175
2 - 8°C
Detection of human HMG2a by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HMG2a antibody BL3259 (Cat. No. A300-736A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).