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Rabbit anti-HMG2a Antibody, Affinity Purified - A300-736A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-HMG2a Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-736A
Name:
Rabbit anti-HMG2a Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to HMG2a immobilized on solid support.The epitope recognized by A300-736A maps to a region between residue 125 and 175 of human High-Mobility Group 2A using the numbering given in entry NP_005333.2 (GeneID 3149).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
high mobility group protein 2a, High mobility group protein 4, high mobility group protein B3, high-mobility group (nonhistone chromosomal) protein 4, HMG2A, HMG-2a, HMG4, HMG-4
Species Reactivity:
Human, Mouse
Immunogen:
Between 125 and 175
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human HMG2a by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HMG2a antibody BL3259 (Cat. No. A300-736A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

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Detection of human and mouse HMG2a by immunohistochemistry. Sample: FFPE section of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-HMG2a (Cat. No. A300-736A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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