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Rabbit anti-HMG2a Antibody, Affinity Purified

Catalogue number:
A300-736A
Size:
20 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to HMG2a immobilized on solid support. The epitope recognized by A300-736A maps to a region between residue 125 and 175 of human High-Mobility Group 2A using the numbering given in entry NP_005333.2 (GeneID 3149). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

high mobility group protein 2a, High mobility group protein 4, high mobility group protein B3, high-mobility group (nonhistone chromosomal) protein 4, HMG2A, HMG-2a, HMG4, HMG-4

Species Reactivity:

Human, Mouse

Immunogen:

between 125 and 175

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human HMG2a by western blot

Detection of human HMG2a by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HMG2a antibody BL3259 (Cat. No. A300-736A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human and mouse HMG2a by im

Detection of human and mouse HMG2a by immunohistochemistry. Sample: FFPE section of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-HMG2a (Cat. No. A300-736A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB