Antibody was affinity purified using an epitope specific to eEF1G immobilized on solid support. The epitope recognized by A300-735A maps to a region between residue 400 and the C-terminus (residue 437) of human Eukaryotic Translation Elongation Factor 1 Gamma using the numbering given in entry NP_001395.1 (GeneID 1937). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
eEF-1B gamma, EF1G, EF-1-gamma, elongation factor 1-gamma, GIG35, pancreatic tumor-related protein, PRO1608, translation elongation factor eEF-1 gamma chain
between 400 and C-term
2 - 8°C
Detection of human and mouse eEF1G by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eEF1G antibody A300-735A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B). eEF1G was immunoprecipitated using rabbit anti-eEF1G antibody A300-734A at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).