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Rabbit anti-eEF1G Antibody, Affinity Purified

Catalogue number:
A300-735A
Size:
20 µg
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Antibody was affinity purified using an epitope specific to eEF1G immobilized on solid support. The epitope recognized by A300-735A maps to a region between residue 400 and the C-terminus (residue 437) of human Eukaryotic Translation Elongation Factor 1 Gamma using the numbering given in entry NP_001395.1 (GeneID 1937). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

eEF-1B gamma, EF1G, EF-1-gamma, elongation factor 1-gamma, GIG35, pancreatic tumor-related protein, PRO1608, translation elongation factor eEF-1 gamma chain

Species Reactivity:

Human, Mouse

Immunogen:

between 400 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse eEF1G by WB

Detection of human and mouse eEF1G by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eEF1G antibody A300-735A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B). eEF1G was immunoprecipitated using rabbit anti-eEF1G antibody A300-734A at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).