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Rabbit anti-eEF1G Antibody, Affinity Purified

Catalogue number:
A300-734A
Size:
20 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to eEF1G immobilized on solid support. The epitope recognized by A300-734A maps to a region between residue 200 and 250 of human Eukaryotic Translation Elongation Factor 1 Gamma using the numbering given in entry NP_001395.1 (GeneID 1937). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Product Type:

Antibodies

Alternative Names:

eEF-1B gamma, EF1G, EF-1-gamma, elongation factor 1-gamma, GIG35, pancreatic tumor-related protein, PRO1608, translation elongation factor eEF-1 gamma chain

Species Reactivity:

Human, Mouse

Immunogen:

between 200 and 250

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse eEF1G by WB

Detection of human and mouse eEF1G by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eEF1G antibody A300-734A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Detection of human eEF1G by immunohistoc

Detection of human eEF1G by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-eEF1G (Cat. No. A300-734A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB