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Bromodomain-Containing (BRD) Antibodies
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Rabbit anti-PP2A Antibody, Affinity Purified - A300-732A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-PP2A Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-732A
Name:
Rabbit anti-PP2A Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to PP2A immobilized on solid support.The epitope recognized by A300-732A maps to a region between residue 260 and the C-terminus (residue 309) of human Protein Phosphatase 2A (PPP2CB, Protein Phosphatase 2, Catalytic Subunit, Beta isoform) using the numbering given in entry NP_004147.1 (GeneID 5516). The region is identical to that of PPP2CA (NP_002706.1; GeneID: 5515)Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
PP2Abeta, PP2A-beta, PP2CB, protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform, protein phosphatase 2, catalytic subunit, beta isoform, protein phosphatase 2, catalytic subunit, beta isozyme, protein phosphatase 2A catalytic subunit, bet
Species Reactivity:
Human, Mouse
Immunogen:
Between 260 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse PP2A by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody A300-732A (lot A300-732A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human PP2A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody A300-732A (lot A300-732A-2) used for IP at 6 µg per reaction. PP2A was also immunoprecipitated by a previous lot of this antibody (A300-732A-1) and rabbit anti-PP2A antibody A300-731A. For blotting immunoprecipitated PP2A, A300-732A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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