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Rabbit anti-PP2A Antibody, Affinity Purified

Catalogue number:
A300-732A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to PP2A immobilized on solid support. The epitope recognized by A300-732A maps to a region between residue 260 and the C-terminus (residue 309) of human Protein Phosphatase 2A (PPP2CB, Protein Phosphatase 2, Catalytic Subunit, Beta isoform) using the numbering given in entry NP_004147.1 (GeneID 5516). The region is identical to that of PPP2CA (NP_002706.1; GeneID: 5515) Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

PP2Abeta, PP2A-beta, PP2CB, protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform, protein phosphatase 2, catalytic subunit, beta isoform, protein phosphatase 2, catalytic subunit, beta isozyme, protein phosphatase 2A catalytic subunit, bet

Species Reactivity:

Human, Mouse

Immunogen:

between 260 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse PP2A by wes

Detection of human and mouse PP2A by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody A300-732A (lot A300-732A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human PP2A by western blot

Detection of human PP2A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody A300-732A (lot A300-732A-2) used for IP at 6 µg per reaction. PP2A was also immunoprecipitated by a previous lot of this antibody (A300-732A-1) and rabbit anti-PP2A antibody A300-731A. For blotting immunoprecipitated PP2A, A300-732A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.