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Rabbit anti-PP2A Antibody, Affinity Purified - A300-731A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-PP2A Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-731A
Name:
Rabbit anti-PP2A Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to PP2A immobilized on solid support.The epitope recognized by A300-731A maps to a region between residue 1 and 50 of human Protein Phosphatase 2A (PPP2CB, Protein Phosphatase 2, Catalytic Subunit, Beta isoform) using the numbering given in entry NP_004147.1 (GeneID 5516). The region is highly conserved with PPP2CA (NP_002706.1; GeneID: 5515)Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
PP2Abeta, PP2A-beta, PP2CB, protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform, protein phosphatase 2, catalytic subunit, beta isoform, protein phosphatase 2, catalytic subunit, beta isozyme, protein phosphatase 2A catalytic subunit, bet
Species Reactivity:
Human, Mouse
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse PP2A by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PP2A antibody BL3130 (Cat. No. A300-731A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). PP2A was also immunoprecipitated using rabbit anti-PP2A antibody BL3131 (Cat. No. A300-732A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

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