Antibody was affinity purified using an epitope specific to XLF immobilized on solid support. The epitope recognized by A300-729A maps to a region between residue 200 and 250 of human XRCC4-Like Factor using the numbering given in entry NP_079058.1 (GeneID 79840). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
non-homologous end-joining factor 1, nonhomologous end-joining factor 1, protein cernunnos, XLF, XRCC4-like factor
between 200 and 250
2 - 8°C
Detection of human XLF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-XLF antibody BL3262 (Cat. No. A300-729A) used for IP at 3 µg/mg lysate. XLF was also immunoprecipitated using rabbit anti-XLF antibody BL3263 (Cat. No. A300-730A) at 3 µg/mg lysate. For blotting immunoprecipitated XLF, BL3263 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human XLF by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- XLF (Cat. No. A300-729A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB