Antibody was affinity purified using an epitope specific to BAAT1 immobilized on solid support. The epitope recognized by A300-728A maps to a region between residue 775 and the C-terminus (residue 821) of human BRCA1-associated protein required for ATM activation-1 (C7orf27) using the numbering given in entry NP_689956.1 (GeneID 221927). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BAAT1, BRCA1-associated ATM activator 1, BRCA1-associated protein required for ATM activation protein 1, C7orf27, HEAT repeat-containing protein C7orf27, NEDCAS, RMFSL
between 775 and C-term
2 - 8°C
Detection of human BAAT1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-BAAT1 antibody A300-728A (lot A300-728A-1) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). BAAT1 was also immunoprecipitated using rabbit anti-BAAT1 antibody BL3282 at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).