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Rabbit anti-Sin3A Antibody, Affinity Purified - A300-725A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Sin3A Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-725A
Name:
Rabbit anti-Sin3A Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Sin3A immobilized on solid support. The epitope recognized by A300-725A maps to a region between residue 375 and 425 of human SIN3 homolog A using the numbering given in entry NP_056292.1 (GeneID 25942). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
histone deacetylase complex subunit Sin3a, paired amphipathic helix protein Sin3a, SIN3 homolog A, transcription regulator, transcriptional corepressor Sin3a, transcriptional co-repressor Sin3A, transcriptional regulator, SIN3A, WITKOS
Species Reactivity:
Human
Immunogen:
between 375 and 425
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Sin3A by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Sin3A antibody A300-725A (lot A300-725A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human Sin3A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Sin3A antibody A300-725A (lot A300-725A-2) used for IP at 6 µg per reaction. Sin3A was also immunoprecipitated by a previous lot of this antibody (lot A300-725A-1) and rabbit anti-Sin3A antibodies A300-724A and A300-726A. For blotting immunoprecipitated Sin3A, A300-725A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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