Rabbit anti-Sin3A Antibody, Affinity Purified
Antibody was affinity purified using an epitope specific to Sin3A immobilized on solid support. The epitope recognized by A300-725A maps to a region between residue 375 and 425 of human SIN3 homolog A using the numbering given in entry NP_056292.1 (GeneID 25942). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Antibodies
histone deacetylase complex subunit Sin3a, paired amphipathic helix protein Sin3a, SIN3 homolog A, transcription regulator, transcriptional corepressor Sin3a, transcriptional co-repressor Sin3A, transcriptional regulator, SIN3A, WITKOS
Human
between 375 and 425
2 - 8°C
Rabbit
Whole IgG
Polyclonal
12352203
Detection of human Sin3A by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Sin3A antibody A300-725A (lot A300-725A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human Sin3A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Sin3A antibody A300-725A (lot A300-725A-2) used for IP at 6 µg per reaction. Sin3A was also immunoprecipitated by a previous lot of this antibody (lot A300-725A-1) and rabbit anti-Sin3A antibodies A300-724A and A300-726A. For blotting immunoprecipitated Sin3A, A300-725A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.