Rabbit anti-Sin3A Antibody, Affinity Purified - A300-725A - 20 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to Sin3A immobilized on solid support. The epitope recognized by A300-725A maps to a region between residue 375 and 425 of human SIN3 homolog A using the numbering given in entry NP_056292.1 (GeneID 25942). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP
Detection of human Sin3A by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Sin3A antibody A300-725A (lot A300-725A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human Sin3A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Sin3A antibody A300-725A (lot A300-725A-2) used for IP at 6 µg per reaction. Sin3A was also immunoprecipitated by a previous lot of this antibody (lot A300-725A-1) and rabbit anti-Sin3A antibodies A300-724A and A300-726A. For blotting immunoprecipitated Sin3A, A300-725A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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