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Rabbit anti-Sin3A Antibody, Affinity Purified

Catalogue number:
A300-725A
Size:
20 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to Sin3A immobilized on solid support. The epitope recognized by A300-725A maps to a region between residue 375 and 425 of human SIN3 homolog A using the numbering given in entry NP_056292.1 (GeneID 25942). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

histone deacetylase complex subunit Sin3a, paired amphipathic helix protein Sin3a, SIN3 homolog A, transcription regulator, transcriptional corepressor Sin3a, transcriptional co-repressor Sin3A, transcriptional regulator, SIN3A, WITKOS

Species Reactivity:

Human

Immunogen:

between 375 and 425

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Sin3A by western blot

Detection of human Sin3A by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Sin3A antibody A300-725A (lot A300-725A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Sin3A by western blot

Detection of human Sin3A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Sin3A antibody A300-725A (lot A300-725A-2) used for IP at 6 µg per reaction. Sin3A was also immunoprecipitated by a previous lot of this antibody (lot A300-725A-1) and rabbit anti-Sin3A antibodies A300-724A and A300-726A. For blotting immunoprecipitated Sin3A, A300-725A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.