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Rabbit anti-Sin3A Antibody, Affinity Purified - A300-724A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Sin3A Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-724A
Name:
Rabbit anti-Sin3A Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to Sin3A immobilized on solid support.The epitope recognized by A300-724A maps to a region between residue 1 and 50 of human SIN3 homolog A using the numbering given in entry NP_056292.1 (GeneID 25942).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
histone deacetylase complex subunit Sin3a, paired amphipathic helix protein Sin3a, SIN3 homolog A, transcription regulator, transcriptional corepressor Sin3a, transcriptional co-repressor Sin3A, transcriptional regulator, SIN3A, WITKOS
Species Reactivity:
Human, Mouse
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse Sin3A by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Sin3A antibody A300-724A (lot A300-724A-1) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Sin3A was also immunoprecipitated using rabbit anti-Sin3A antibodies A300-725A, BL2730 and BL2731 at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

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Detection of human and mouse Sin3A by immunohistochemistry. Sample: FFPE sections of human metastatic lymph node (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Sin3A (Cat. No. A300-724A Lot1) used at a dilution of 1:200 (1 µg/ml). Detection: DAB

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