Antibody was affinity purified using an epitope specific to PRMT1 immobilized on solid support. The epitope recognized by A300-723A maps to a region between residue 300 and the C-terminus (residue 361) of human Protein Arginine N-Methyltransferase 1 using the numbering given in entry NP_001527.2 (GeneID 3276). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ANM1, HCP1, heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 2, histone-arginine N-methyltransferase PRMT1, HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2, HRMT1L2, interferon receptor 1-bound protein 4, IR1B4, protein arginine N-met
between 300 and C-term
2 - 8°C
Detection of human and mouse PRMT1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PRMT1 antibody A300-723A (lot A300-723A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human PRMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer + 0.3% SDS, 1.5% sodium deoxycholate. Antibodies: Affinity purified rabbit anti-PRMT1 antibody A300-723A (lot A300-723A-2) used for IP at 3 µg per reaction. PRMT1 was also immunoprecipitated by rabbit anti-PRMT1 antibody A300-722A. For blotting immunoprecipitated PRMT1, A300-723A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.