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Rabbit anti-PRMT1 Antibody, Affinity Purified

Catalogue number:
A300-723A
Size:
20 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to PRMT1 immobilized on solid support. The epitope recognized by A300-723A maps to a region between residue 300 and the C-terminus (residue 361) of human Protein Arginine N-Methyltransferase 1 using the numbering given in entry NP_001527.2 (GeneID 3276). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

ANM1, HCP1, heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 2, histone-arginine N-methyltransferase PRMT1, HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2, HRMT1L2, interferon receptor 1-bound protein 4, IR1B4, protein arginine N-met

Species Reactivity:

Human, Mouse

Immunogen:

between 300 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse PRMT1 by we

Detection of human and mouse PRMT1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PRMT1 antibody A300-723A (lot A300-723A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human PRMT1 by western blot

Detection of human PRMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer + 0.3% SDS, 1.5% sodium deoxycholate. Antibodies: Affinity purified rabbit anti-PRMT1 antibody A300-723A (lot A300-723A-2) used for IP at 3 µg per reaction. PRMT1 was also immunoprecipitated by rabbit anti-PRMT1 antibody A300-722A. For blotting immunoprecipitated PRMT1, A300-723A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human PRMT1 by immunohistoc

Detection of human PRMT1 by immunohistochemistry. Sample: FFPE section of human head and neck squamous cell carcinoma. Antibody: Affinity purified rabbit anti- PRMT1 (Cat. No. A300-723A Lot2) used at a dilution of 1:1000 (0.2 µg/ml). Detection: DAB

Detection of mouse PRMT1 by immunohistoc

Detection of mouse PRMT1 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- PRMT1 (Cat. No. A300-723A Lot2) used at a dilution of 1:200 (1 µg/ml). Detection: DAB