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Highly effective & efficient in vitro delivery of Cas9 RNP for genome editing in challenging cells

Rabbit anti-PRMT1 Antibody, Affinity Purified - A300-723A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-PRMT1 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-723A
Name:
Rabbit anti-PRMT1 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to PRMT1 immobilized on solid support.The epitope recognized by A300-723A maps to a region between residue 300 and the C-terminus (residue 361) of human Protein Arginine N-Methyltransferase 1 using the numbering given in entry NP_001527.2 (GeneID 3276).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
ANM1, HCP1, heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 2, histone-arginine N-methyltransferase PRMT1, HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2, HRMT1L2, interferon receptor 1-bound protein 4, IR1B4, protein arginine N-met
Species Reactivity:
Human, Mouse
Immunogen:
Between 300 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse PRMT1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PRMT1 antibody A300-723A (lot A300-723A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human PRMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer + 0.3% SDS, 1.5% sodium deoxycholate. Antibodies: Affinity purified rabbit anti-PRMT1 antibody A300-723A (lot A300-723A-2) used for IP at 3 µg per reaction. PRMT1 was also immunoprecipitated by rabbit anti-PRMT1 antibody A300-722A. For blotting immunoprecipitated PRMT1, A300-723A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

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Detection of human PRMT1 by immunohistochemistry. Sample: FFPE section of human head and neck squamous cell carcinoma. Antibody: Affinity purified rabbit anti- PRMT1 (Cat. No. A300-723A Lot2) used at a dilution of 1:1000 (0.2 µg/ml). Detection: DAB

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Detection of mouse PRMT1 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- PRMT1 (Cat. No. A300-723A Lot2) used at a dilution of 1:200 (1 µg/ml). Detection: DAB

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