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Rabbit anti-RNF40 Antibody, Affinity Purified - A300-719A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-RNF40 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-719A
Name:
Rabbit anti-RNF40 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to RNF40 immobilized on solid support.The epitope recognized by A300-719A maps to a region between residue 540 and 590 of human Ring Finger 40 using the numbering given in entry NP_055586.1 (GeneID 9810).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
95 kDa retinoblastoma protein binding protein, 95 kDa retinoblastoma-associated protein, BRE1 E3 ubiquitin ligase homolog B, BRE1B, BRE1-B, E3 ubiquitin-protein ligase BRE1B, Rb-associated protein, RBP95, RING finger protein 40, ring finger protein 40, E3
Species Reactivity:
Human
Immunogen:
Between 540 and 590
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human RNF40 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RNF40 antibody BL3314 (Cat. No. A300-719A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). RNF40 was also immunoprecipitated using rabbit anti-RNF40 antibodies BL3315 (Cat. No. A300-720A) and BL3316 at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

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Detection of human RNF40 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- RNF40 (Cat. No. A300-719A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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