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Rabbit anti-NUP214 Antibody, Affinity Purified - A300-716A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-NUP214 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-716A
Name:
Rabbit anti-NUP214 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to NUP214 immobilized on solid support.The epitope recognized by A300-716A maps to a region between residue 1250 and 1300 of human Nuceloporin 214 using the numbering given in entry NP_005076.3 (GeneID 8021).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
214 kDa nucleoporin, CAIN, CAN, CAN protein, putative oncogene, nuclear pore complex protein Nup214, nucleoporin 214kDa, Nucleoporin Nup214, Protein CAN
Species Reactivity:
Human
Immunogen:
Between 1250 and 1300
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse NUP214 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), and HEK293T (50µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP214 antibody A300-716A (lot A300-716A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human NUP214 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NUP214 antibody A300-716A (lot A300-716A-2) used for IP at 6 µg per reaction. NUP214 was also immunoprecipitated by a previous lot of this antibody (lot A300-716A-1) and rabbit anti-NUP214 antibody A300-717A For blotting immunoprecipitated NUP214, A300-716A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human NUP214 by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Affinity purified rabbit anti- NUP214 (Cat. No. A300-716A Lot2) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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Detection of human NUP214 by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Affinity purified rabbit anti- NUP214 (Cat. No. A300-716A Lot2) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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