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Rabbit anti-RNF20 Antibody, Affinity Purified

Catalogue number:
A300-715A
Size:
100 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to RNF20 immobilized on solid support. The epitope recognized by A300-715A maps to a region between residue 125 and 175 of human Ring Finger 20 using the numbering given in entry NP_062538.5 (GeneID 56254). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

BRE1, BRE1 E3 ubiquitin ligase homolog, BRE1A, BRE1-A, E3 ubiquitin-protein ligase BRE1A, hBRE1, homolog of S. cerevisiae BRE1, RING finger protein 20, RING-type E3 ubiquitin transferase BRE1A

Species Reactivity:

Human, Mouse

Immunogen:

between 125 and 175

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human RNF20 by western blot

Detection of human RNF20 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RNF20 antibody A300-715A (lot A300-715A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human RNF20 by western blot

Detection of human RNF20 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNF20 antibody A300-715A (lot A300-715A-2) used for IP at 6 µg per reaction. RNF20 was also immunoprecipitated by rabbit anti-RNF20 antibody A300-714A. For blotting immunoprecipitated RNF20, A300-715A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human RNF20 by immunohistoc

Detection of human RNF20 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RNF20 (Cat. No. A300-715A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

Detection of mouse RNF20 by immunohistoc

Detection of mouse RNF20 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- RNF20 (Cat. No. A300-715A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB