Antibody was affinity purified using an epitope specific to Nucleolin (NCL) immobilized on solid support. The epitope recognized by A300-709A maps to a region between residue 250 and 300 of human Nucleolin using the numbering given in entry NP_005372.2 (GeneID 4691). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
C23, Nsr1, nucleolin, Protein C23
between 250 and 300
2 - 8°C
Detection of human Nucleolin by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Nucleolin antibody BL3213 (Cat. No. A300-709A) used for WB at 0.02 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Nucleolin was also immunoprecipitated using rabbit anti-Nucleolin antibodies BL3214 (Cat. No. A300-710A) and BL3215 (Cat. No. A300-711A). Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Detection of human Nucleolin by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti- Nucleolin (Cat. No. A300-709A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB