Antibody was affinity purified using an epitope specific to Sirt2 immobilized on solid support. The epitope recognized by A300-702A maps to a region between residue 1 and 50 of human sirtuin (silent mating type information regulator 2 homolog) 2 using the numbering given in entry NP_036369.2 (GeneID 22933). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
NAD-dependent deacetylase sirtuin-2, NAD-dependent protein deacetylase sirtuin-2, regulatory protein SIR2 homolog 2, silent information regulator 2, SIR2, SIR2L, SIR2L2, SIR2-like protein 2, sir2-related protein type 2, sirtuin type 2
between 1 and 50
2 - 8°C
Detection of human Sirt2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from nocodazole treated HeLa cells. Antibodies: Affinity purified rabbit anti-Sirt2 antibody A300-702A (lot A700-702A-1) used for WB at 1 µg/ml and used for immunoprecipitation at 3 µg/mg lysate. Sirt2 was also immunoprecipitated by rabbit anti-Sirt2 antibody BL3199. Detection: Chemiluminescence with an exposure time of 30 seconds.