Rabbit anti-PTEN Antibody, Affinity Purified - A300-700A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to PTEN immobilized on solid support. The epitope recognized by A300-700A maps to a region between residue 325 and 375 of human phosphatase and tensin homolog using the numbering given in entry NP_000305.2 (GeneID 5728). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
IP
Detection of human PTEN by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PTEN antibody A300-700A (lot A300-700A-1) used for immunoprecipitation at 3 µg/mg lysate. PTEN was also immunoprecipitated by rabbit anti-PTEN antibodies BL2788, BL2789 and A300-701A. For blotting immunoprecipitated PTEN, A300-701A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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