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Rabbit anti-PTEN Antibody, Affinity Purified

Catalogue number:
A300-700A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to PTEN immobilized on solid support. The epitope recognized by A300-700A maps to a region between residue 325 and 375 of human phosphatase and tensin homolog using the numbering given in entry NP_000305.2 (GeneID 5728). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP
Product Type:

Antibodies

Alternative Names:

10q23del, BZS, CWS1, DEC, GLM2, MHAM, mitochondrial phosphatase and tensin protein alpha, mitochondrial PTENalpha, MMAC1, MMAC1 phosphatase and tensin homolog deleted on chromosome 10, mutated in multiple advanced cancers 1, Phosphatase and tensin homolog

Species Reactivity:

Human

Immunogen:

between 325 and 375

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human PTEN by western blot

Detection of human PTEN by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PTEN antibody A300-700A (lot A300-700A-1) used for immunoprecipitation at 3 µg/mg lysate. PTEN was also immunoprecipitated by rabbit anti-PTEN antibodies BL2788, BL2789 and A300-701A. For blotting immunoprecipitated PTEN, A300-701A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.