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Rabbit anti-DDX24 Antibody, Affinity Purified

Catalogue number:
A300-697A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to DDX24 immobilized on solid support. The epitope recognized by A300-697A maps to a region between residue 325 and 375 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 24 using the numbering given in entry NP_065147.1 (GeneID 57062). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

ATP-dependent RNA helicase DDX24, DEAD (Asp-Glu-Ala-Asp) box helicase 24, DEAD (Asp-Glu-Ala-Asp) box polypeptide 24, DEAD box protein 24, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 24, S. cerevisiae CHL1-like helicase

Species Reactivity:

Human

Immunogen:

between 325 and 375

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human DDX24 by western blot

Detection of human DDX24 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T and NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX24 antibody A300-697A (lot A300-697A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human DDX24 by western blot

Detection of human DDX24 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX24 antibody A300-697A (lot A300-697A-2) used for IP at 6 µg per reaction. DDX24 was also immunoprecipitated by a previous lot of this antibody (lot A300-697A-1). For blotting immunoprecipitated DDX24, A300-697A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.