Antibody was affinity purified using an epitope specific to DDX24 immobilized on solid support. The epitope recognized by A300-696A maps to a region between residue 1 and 50 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 24 using the numbering given in entry NP_065147.1 (GeneID 57062). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent RNA helicase DDX24, DEAD (Asp-Glu-Ala-Asp) box helicase 24, DEAD (Asp-Glu-Ala-Asp) box polypeptide 24, DEAD box protein 24, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 24, S. cerevisiae CHL1-like helicase
between 1 and 50
2 - 8°C
Detection of human DDX24 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DDX24 antibody BL3136 (Cat. No. A300-696A) used for WB at 0.04 µg (A) and 1 µg/ml (B) and used for immunoprecipitation at 3 µg/mg lysate (B). DDX24 was also immunoprecipitated by rabbit anti-DDX24 antibody BL3138 (Cat. No. A300-697A) and BL3139 (Cat. No. A300-698A). Detection: Chemiluminescence with exposure times of 3 seconds (A and B).