Antibody was affinity purified using an epitope specific to DDX23 immobilized on solid support. The epitope recognized by A300-695A maps to a region between residue 775 and the C-terminus (residue 820) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 using the numbering given in entry NP_004809.2 (GeneID 9416). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
100 kDa U5 snRNP-specific protein, DEAD (Asp-Glu-Ala-Asp) box polypeptide 23, DEAD box protein 23, probable ATP-dependent RNA helicase DDX23, prp28, PRP28 homolog, PRP28 homolog, yeast, PRP28p homolog, PRPF28, SNRNP100, U5 snRNP 100 kD protein, U5-100K, U
between 775 and C-term
2 - 8°C
Detection of human and mouse DDX23 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DDX23 antibody A300-695A (lot A300-695A-1) used for WB at 0.04 µg (A) and 1 µg/ml (B) and used for immunoprecipitation at 3 µg/mg lysate (B). DDX23 was also immunoprecipitated by rabbit anti-DDX23 antibody A300-693A but not by BL3134. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).