*

Rabbit anti-DDX23 Antibody, Affinity Purified

Catalogue number:
A300-695A
Size:
20 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£322.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to DDX23 immobilized on solid support. The epitope recognized by A300-695A maps to a region between residue 775 and the C-terminus (residue 820) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 using the numbering given in entry NP_004809.2 (GeneID 9416). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

100 kDa U5 snRNP-specific protein, DEAD (Asp-Glu-Ala-Asp) box polypeptide 23, DEAD box protein 23, probable ATP-dependent RNA helicase DDX23, prp28, PRP28 homolog, PRP28 homolog, yeast, PRP28p homolog, PRPF28, SNRNP100, U5 snRNP 100 kD protein, U5-100K, U

Species Reactivity:

Human, Mouse

Immunogen:

between 775 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse DDX23 by we

Detection of human and mouse DDX23 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DDX23 antibody A300-695A (lot A300-695A-1) used for WB at 0.04 µg (A) and 1 µg/ml (B) and used for immunoprecipitation at 3 µg/mg lysate (B). DDX23 was also immunoprecipitated by rabbit anti-DDX23 antibody A300-693A but not by BL3134. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).