Antibody was affinity purified using an epitope specific to CDX2 immobilized on solid support. The epitope recognized by A300-692A maps to a region between residue 275 and the C-terminus (residue 313) of human Caudal Type Homeobox Transcription Factor 2 using the numbering given in entry NP_001256.1 (GeneID 1045). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
caudal type homeobox transcription factor 2, caudal-type homeobox protein 2, CDX2/AS, CDX3, CDX-3, homeobox protein CDX-2, homeobox protein miniCDX2
between 275 and C-term
2 - 8°C
Detection of human CDX2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CDX2 antibody BL3195 (Cat. No. A300-692A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. CDX2 was also immunoprecipitated by rabbit anti-CDX2 antibody BL3194 (Cat. No. A300-691A) using 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).