Antibody was affinity purified using an epitope specific to hnRNP-U immobilized on solid support. The epitope recognized by A300-690A maps to a region between residue 775 and the C-terminus (residue 824) of human Heterogeneous Nuclear Ribonucleoprotein U using the numbering given in entry NP_114032.1 (GeneID 3192). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: WB, IP
EIEE54, GRIP120, heterogeneous nuclear ribonucleoprotein U, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), hnRNP U, HNRNPU antisense RNA 1, HNRNPU-AS1, HNRPU, nuclear p120 ribonucleoprotein, p120, p120 nuclear protein, pp120, SA
between 775 and C-term
2 - 8°C
Detection of human and mouse hnRNP-U by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-hnRNP-U antibody BL2765 (Cat. No. A300-690A) used for WB at 0.4 µg/ml (A) and used for IP at 3 µg/mg lysate. immunoprecipitatesd hnRNP-U was blotted using rabbit anti-hnRNP-U antibody BL2763 (Cat. No. A300-689A) at 1 µg/ml. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 30 seconds (B).