Antibody was affinity purified using an epitope specific to RPL26 immobilized on solid support. The epitope recognized by A300-685A maps to a region between residue 1 and 50 of human Ribosomal Protein L26 using the numbering given in entry NP_000978.1 (GeneID 6154). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
60S ribosomal protein L26, DBA11, L26, large ribosomal subunit protein uL24
between 1 and 50
2 - 8°C
Detection of human and mouse RPL26 by western blot (h and m) and immunoprecipitation (m). Samples: Whole cell lysate from HeLa (H; 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RPL26 antibody BL3208 (Cat. No. A300-685A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. RPL26 was also immunoprecipitated by rabbit anti-RPL26 antibody BL3211 (Cat. No. A300-686A). Detection: Chemiluminescence with exposure times of 3 minutes (A) 30 seconds (B).