Antibody was affinity purified using an epitope specific to MAML3 immobilized on solid support. The epitope recognized by A300-683A maps to a region between residue 150 and 200 of human Mastermind-Like 3 using the numbering given in entry NP_061187.2 (GeneID 55534). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CAG repeat containing (glia-derived nexin I alpha), CAGH3, ERDA3, expanded repeat domain, CAG/CTG 3, GDN, MAM2, MAM-2, mam-3, mastermind-like protein 3, polyglutamine rich, TNRC3, trinucleotide repeat containing 3
between 150 and 200
2 - 8°C
Detection of human and mouse MAML3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAML3 antibody A300-683A (lot A300-683A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human MAML3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAML3 antibody A300-683A (lot A300-683A-2) used for IP at 6 µg per reaction. MAML3 was also immunoprecipitated by rabbit anti-MAML3 antibody A300-684A. For blotting immunoprecipitated MAML3, A300-683A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.