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Rabbit anti-hnRNP-K Antibody, Affinity Purified - A300-675A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-hnRNP-K Antibody, Affinity Purified
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Size: 20 µg
£302.00
GBP | Euro | USD
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Catalogue number:
A300-675A
Name:
Rabbit anti-hnRNP-K Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to hnRNP-K immobilized on solid support.The epitope recognized by A300-675A maps to a region between residue 50 and 100 of human Heterogeneous Nuclear Ribonucleoprotein K using the numbering given in entries NP_002131.2 for hnRNP-K isoform a and NP_112552.1 for hnRNP-K isoform b (GeneID 3190).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
AUKS, CSBP, dC-stretch binding protein, heterogeneous nuclear ribonucleoprotein K, hnRNP K, HNRPK, transformation upregulated nuclear protein, Transformation up-regulated nuclear protein, TUNP
Species Reactivity:
Human, Mouse
Immunogen:
Between 50 and 100
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse hnRNP-K by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-hnRNP-K antibody BL3184 (Cat. No. A300-675A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. immunoprecipitation reactions were also performed using antibodies BL3183 (Cat. No. A300-674A), BL3185 (Cat. No. A300-676A), BL3186 (Cat. No. A300-677A) and BL3187 (Cat. No. A300-678A). Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

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Detection of human and mouse hnRNP-K by immunohistochemistry. Sample: FFPE section of human skin carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-hnRNP-K (Cat. No. A300-675A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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