www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Rabbit anti-hnRNP-K Antibody, Affinity Purified

Catalogue number:
A300-674A
Size:
20 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£322.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to hnRNP-K immobilized on solid support. The epitope recognized by A300-674A maps to a region between residue 1 and 50 of human Heterogeneous Nuclear Ribonucleoprotein K using the numbering given in entries NP_002131.2 for hnRNP-K isoform a and NP_112552.1 for hnRNP-K isoform b (GeneID 3190). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

AUKS, CSBP, dC-stretch binding protein, heterogeneous nuclear ribonucleoprotein K, hnRNP K, HNRPK, transformation upregulated nuclear protein, Transformation up-regulated nuclear protein, TUNP

Species Reactivity:

Human, Mouse

Immunogen:

between 1 and 50

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse hnRNP-K by

Detection of human and mouse hnRNP-K by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-hnRNP-K antibody BL3183 (Cat. No. A300-674A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. immunoprecipitation reactions were also performed using antibodies BL3184 (Cat. No. A300-675A), BL3185 (Cat. No. A300-676A), BL3186 (Cat. No. A300-677A) and BL3187 (Cat. No. A300-678A). Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Detection of human and mouse hnRNP-K by

Detection of human and mouse hnRNP-K by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-hnRNP-K (Cat. No. A300-674A Lot1) used at a dilution of 1:1,000 (0.2µg/ml). Detection: DAB