Antibody was affinity purified using an epitope specific to MAML1 immobilized on solid support. The epitope recognized by A300-673A maps to a region between residue 1000 and the C-terminus (residue 1042) of human Mastermind-like 1 using the numbering given in entry NP_055572.1 (GeneID 9794). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Mam1, Mam-1, mastermind homolog, mastermind-like 1, mastermind-like protein 1
between 1000 and C-term
2 - 8°C
Detection of human MAML1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-MAML1 antibody BL1239 (Cat. No. A300-673A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and for IP at 3 µg/mg lysate. MAML1 was also immunoprecipitated by rabbit anti-MAML1 antibodies BL1237 (Cat. No. A300-671A) and BL1238 (Cat. No. A300-672A) using 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds (A and B).
Detection of human MAML1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MAML1 (Cat. No. A300-673A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB