Antibody was affinity purified using an epitope specific to CERT/GPBP immobilized on solid support. The epitope recognized by A300-669A maps to a region between residue 300 and 350 of human CERT/GPBP using the numbering given in entry NP_005704.1 (GeneID 10087). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ceramide transfer protein, ceramide transporter, CERT, CERTL, collagen type IV alpha-3-binding protein, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein, Goodpasture antigen-binding protein, GPBP, hCERT, lipid-transfer protein CERTL, MRD34
between 300 and 350
2 - 8°C
Detection of human CERT(GPBP) by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CERT(GPBP) antibody BL2222 (Cat. No. A300-669A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. CERT(GPBP) was also immunoprecipitated using rabbit anti-CERT(GPBP) antibody BL2221 (Cat. No. A300-668A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).