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Rabbit anti-Tip41 Antibody, Affinity Purified - A300-663A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Tip41 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-663A
Name:
Rabbit anti-Tip41 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Tip41 immobilized on solid support.The epitope recognized by A300-663A maps to a region between residue 225 and the C-terminus (residue 272) of human TIP41, TOR-signaling Pathway Regulator-like using the numbering given in entry NP_690866.1 (GeneID 261726).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
putative MAPK-activating protein PM10, TIP, TIP41, TIP41, TOR signaling pathway regulator-like, TIP41-like protein, TIPRL1, type 2A-interacting protein
Species Reactivity:
Human, Mouse
Immunogen:
Between 225 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse Tip41 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP; 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Tip41 antibody BL3057 (Cat. No. A300-663A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Efficiency of immunoprecipitation of Tip41 was much less when using rabbit anti-Tip41 antibody BL3056 (Cat. No. A300-662A) or BL3058. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).

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Detection of human Tip41 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- Tip41 (Cat. No. A300-663A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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