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Rabbit anti-REA Antibody, Affinity Purified - A300-658A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-REA Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-658A
Name:
Rabbit anti-REA Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to REA immobilized on solid support.The epitope recognized by A300-658A maps to a region between residue 250 and the C-terminus (residue 299) of human Repressor of Estrogen Activity (Prohibitin 2) using the numbering given in entry NP_009204.1 (GeneID 11331).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
BAP, Bap37, BCAP37, B-cell associated protein, B-cell receptor-associated protein BAP37, D-prohibitin, hBAP, p22, PNAS-141, prohibitin-2, REA, repressor of estrogen receptor activity
Species Reactivity:
Human, Mouse
Immunogen:
Between 250 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse REA by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T, 50 µg), and mouse NIH 3T3 (M, 50 µg) cells. Antibodies: Affinity purified rabbit anti-REA antibody BL1707 (Cat. No. A300-658A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. REA was also immunoprecipitated using rabbit anti-REA antibodies BL1704 (Cat. No. A300-656A) and BL1706 (Cat. No. A300-657A), each of which recognizes a different epitope on REA. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

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