Antibody was affinity purified using an epitope specific to Phospho 53BP1 immobilized on solid support. Immunogen for A300-652A was a phosphorylated synthetic peptide, which represented a portion of human p53 Binding Protein 1 (GeneID 7158) around serine 25 according to the numbering given in entry NP_005648.1. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
53BP1, p202, p53-binding protein 1, p53BP1, TDRD30, TP53-binding protein 1, tumor protein 53-binding protein, 1, tumor suppressor p53-binding protein 1
surrounding serine 25
2 - 8°C
Detection of Phospho 53BP1 (S25) by western blot. Samples: Whole cell lysate (30 µg or 15 µg for WB; 1 mg for IP, 20% of IP loaded) from HEK293T cells that were mock treated or treated with NCS (200 ng/ml; 1h). Antibodies: Affinity purified rabbit anti-Phospho 53BP1 antibody A300-652A (lot no. A300-652A-2) at 0.04 µg/ml (A) and 0.1 µg/ml (B) for WB. 53BP1 was immunoprecipitated using affinity purified rabbit anti-53BP1 antibody A300-272A. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).