Antibody was affinity purified using an epitope specific to DDX18 immobilized on solid support. The epitope recognized by A300-636A maps to a region between residue 625 and the C-terminus (residue 670) of human DEAD (Asp-Glu-Ala-Asp) Box Protein 18 using the numbering given in entry NP_006764.3 (GeneID 8886). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent RNA helicase DDX18, DEAD (Asp-Glu-Ala-Asp) box polypeptide 18, DEAD box protein 18, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 18 (Myc-regulated), Has1, MrDb, Myc-regulated DEAD box protein
between 625 and C-term
2 - 8°C
Detection of human DDX18 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg for WB) cells. Antibodies: Affinity purified rabbit anti-DDX18 antibody BL3018 (Cat. No. A300-636A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and for IP at 3 µg/mg lysate. DDX18 was also immunoprecipitated using rabbit anti-DDX18 antibody BL3015 (Cat. No. A300-635A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).