Antibody was affinity purified using an epitope specific to BLAP75 immobilized on solid support. The epitope recognized by A300-631A maps to a region between residues 275 and 300 of human BLM Associated Protein, 75kDA using the numbering given in entry NP_079221.1 (GeneID 80010). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BLAP75, BLM-associated polypeptide, 75 kDa, BLM-associated protein 75 kDa, BLM-associated protein of 75 kDa, C9orf76, FAAP75, homolog of yeast RecQ-mediated genome instability 1 (RMI1), recQ-mediated genome instability protein 1, RMI1, RecQ mediated genom
between 275 and 300
2 - 8°C
Detection of human BLAP75 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BLAP75 antibody BL3007 (Cat. No. A300-631A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). BLAP75 was also immunoprecipitated using rabbit anti-BLAP75 antibodies BL3006 and BL3008. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of human BLAP75 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BLAP75 (Cat. No. A300-631A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB