Antibody was affinity purified using an epitope specific to DDX21 immobilized on solid support. The epitope recognized by A300-629A maps to a region between residue 725 and the C-terminus (residue 783) of human DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 21 using the numbering given in entry NP_004719.2 (GeneID 9188). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DEAD (Asp-Glu-Ala-Asp) box helicase 21, DEAD (Asp-Glu-Ala-Asp) box polypeptide 21, DEAD box protein 21, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21, DEAD-box helicase 21, Gu protein, GUA, gu-alpha, GURDB, nucleolar RNA helicase 2, nucleolar RNA helica
between 725 and C-term
2 - 8°C
Detection of human DDX21 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX21 antibody A300-629A (lot A300-629A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human DDX21 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX21 antibody A300-629A (lot A300-629A-2) used for IP at 6 µg per reaction. DDX21 was also immunoprecipitated by rabbit anti-DDX21 antibodies A300-627A and A300-628A. For blotting immunoprecipitated DDX21, A300-629A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.