Antibody was affinity purified using an epitope specific to DDX21 immobilized on solid support. The epitope recognized by A300-628A maps to a region between residues 50 and 100 of human DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 21 using the numbering given in entry NP_004719.2 (GeneID 9188). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DEAD (Asp-Glu-Ala-Asp) box helicase 21, DEAD (Asp-Glu-Ala-Asp) box polypeptide 21, DEAD box protein 21, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21, DEAD-box helicase 21, Gu protein, GUA, gu-alpha, GURDB, nucleolar RNA helicase 2, nucleolar RNA helica
between 50 and 100
2 - 8°C
Detection of human DDX21 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX21 antibody A300-628A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. DDX21 was also immunoprecipitated using rabbit anti-DDX21 antibodies A300-627A and A300-629A at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).