Antibody was affinity purified using an epitope specific to DHX8 immobilized on solid support. The epitope recognized by A300-624A maps to a region between residues 325 and 375 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 using the numbering given in entry NP_004932.1 (GeneID 1659). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent RNA helicase DHX8, DDX8, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase), DEAH (Asp-Glu-Ala-His) box polypeptide 8, DEAH box protein 8, Dhr2, HRH1, PRP22, PRPF22, RNA helicase HRH1
between 325 and 375
2 - 8°C
Detection of human DHX8 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX8 antibody A300-624A (lot A300-624A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human DHX8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DHX8 antibody A300-624A (lot A300-624A-2) used for IP at 3 µg per reaction. DHX8 was also immunoprecipitated by rabbit anti-DHX8 antibody A300-625A. For blotting immunoprecipitated DHX8, A300-624A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.