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Rabbit anti-DHX8 Antibody, Affinity Purified - A300-624A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-DHX8 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-624A
Name:
Rabbit anti-DHX8 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to DHX8 immobilized on solid support. The epitope recognized by A300-624A maps to a region between residues 325 and 375 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 using the numbering given in entry NP_004932.1 (GeneID 1659).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
ATP-dependent RNA helicase DHX8, DDX8, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase), DEAH (Asp-Glu-Ala-His) box polypeptide 8, DEAH box protein 8, Dhr2, HRH1, PRP22, PRPF22, RNA helicase HRH1
Species Reactivity:
Human, Mouse
Immunogen:
Between 325 and 375
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human DHX8 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX8 antibody A300-624A (lot A300-624A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human DHX8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DHX8 antibody A300-624A (lot A300-624A-2) used for IP at 3 µg per reaction. DHX8 was also immunoprecipitated by rabbit anti-DHX8 antibody A300-625A. For blotting immunoprecipitated DHX8, A300-624A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human DHX8 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody:Affinity purified rabbit anti-DHX8 antibody (A300-624A lot 2) used at 1:1000 (0.2 µg/ml). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).

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Detection of mouse DHX8 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody:Affinity purified rabbit anti-DHX8 antibody (A300-624A lot 2) used at 1:1000 (0.2 µg/ml). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).

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