Antibody was affinity purified using an epitope specific to ErbB2 immobilized on solid support. The epitope recognized by A300-623A maps to a region between residue 1200 and the C-terminus (residue 1255) of human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 using the numbering given in entry NP_004439.2 (GeneID 2064). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CD antigen CD340, CD340, c-erb B2/neu protein, HER2, HER-2, HER-2/neu, herstatin, human epidermal growth factor receptor 2, metastatic lymph node gene 19 protein, MLN 19, NEU, neuro/glioblastoma derived oncogene homolog, neuroblastoma/glioblastoma derived
between 1200 and C-term
2 - 8°C
Detection of human and mouse ErbB2 by western blot (H & M) and immunoprecipitation (H). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg for WB) and mouse NIH 3T3 (M; 50 µg for WB) cells. Antibodies: Affinity purified rabbit anti-ErbB2 antibody BL3033 (Cat. No. A300-623A) used for WB at 1 µg/ml (A) and 0.04 µg/ml (B) and used for IP at 3 µg/mg lysate. ErbB2 was also immunoprecipitated using rabbit anti-ErbB2 antibodies BL3030 (Cat. No. A300-621A) BL3031 and BL3032 (Cat. No. A300-622A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Detection of human ErbB2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ErbB2 (Cat. No. A300-623A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB