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Rabbit anti-DDX10 Antibody, Affinity Purified

Catalogue number:
A300-617A
Size:
20 µg
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Antibody was affinity purified using an epitope specific to DDX10 immobilized on solid support. The epitope recognized by A300-617A maps to a region between residue 825 and the C-terminus (residue 875) of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 10 using the numbering given in entry NP_004389.2 (GeneID 1662). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Detection of human DDX10 by western blot
Product Type:

Antibodies

Alternative Names:

Dbp4, DDX10-NUP98 fusion protein type 2, DEAD (Asp-Glu-Ala-Asp) box polypeptide 10, DEAD box protein 10, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 10 (RNA helicase), HRH-J8, probable ATP-dependent RNA helicase DDX10

Species Reactivity:

Human

Immunogen:

between 825 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human DDX10 by western blot

Detection of human DDX10 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DDX10 antibody BL3004 (Cat. No. A300-617A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. DDX10 was also immunoprecipitated with rabbit anti-DDX10 antibody BL3003 (Cat. No. A300-616A) used at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).