Antibody was affinity purified using an epitope specific to DDX10 immobilized on solid support. The epitope recognized by A300-617A maps to a region between residue 825 and the C-terminus (residue 875) of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 10 using the numbering given in entry NP_004389.2 (GeneID 1662). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: WB, IP
Dbp4, DDX10-NUP98 fusion protein type 2, DEAD (Asp-Glu-Ala-Asp) box polypeptide 10, DEAD box protein 10, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 10 (RNA helicase), HRH-J8, probable ATP-dependent RNA helicase DDX10
between 825 and C-term
2 - 8°C
Detection of human DDX10 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DDX10 antibody BL3004 (Cat. No. A300-617A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. DDX10 was also immunoprecipitated with rabbit anti-DDX10 antibody BL3003 (Cat. No. A300-616A) used at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).