Antibody was affinity purified using an epitope specific to SKIV2L2 immobilized on solid support. The epitope recognized by A300-615A maps to a region between residues 650 and 700 of human SuperKiller Viralicidic Activity 2-Like 2 using the numbering given in entry NP_056175.2 (GeneID 23517). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent helicase SKIV2L2, ATP-dependent RNA helicase DOB1, ATP-dependent RNA helicase SKIV2L2, Dob1, exosome RNA helicase MTR4, fSAP118, functional spliceosome-associated protein 118, KIAA0052, Mtr4, Ski2 like RNA helicase 2, SKIV2L2, superkiller vi
between 650 and 700
2 - 8°C
Detection of human SKIV2L2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SKIV2L2 antibody A300-615A (lot A300-615A-2) used for IP at 6 µg per reaction. For blotting immunoprecipitated SKIV2L2, rabbit anti-SKIV2L2 antibody A300-614A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.